Bio-One Inc. is the category-leader and first in biohazard and decontamination cleanup services growing quickly to 100+ franchise owners operating 130+ locations. Helping people and communities in their greatest times of need, Bio-one answers the call with discretion, care, and compassion.
Bio-One believes in a simple and clear mission: Help First, Business Second. A close-knit brand of community heroes devoted to cleaning up what others won’t (or can’t) including biohazard cleanup, crime scene cleanup, hoarding cleanup, mold remediation, and more. Bio-One franchisees are trained to handle these situations with compassion and professionalism, providing a valuable service to their communities.
Acquired by Five Star Franchising in 2021, Bio-One is well-positioned and supported for rapid expansion and growth across North America. With low start-up costs and high profit margins, this recession-resilient industry provides an opportunity for strong leaders who are passionate about making a difference in their community.Franchise Service Lines:
- Crime scene cleanup
- Suicide cleanup
- Blood spill cleanup
- Hoarding cleanup
- Virus and bacterial disinfection
- Decomposition and undiscovered death
- Emergency vehicle decontamination
- Odor removal
- Teargas cleanup
- Rodent droppings
- Feces and urine cleanup
- Homeless encampment cleanup
- Biorecovery mold remediation
- Medical waste
Key Differentiators:
- First to Franchise Category Leader
- Niche specialized services in demand
- Purpose-Driven Team and Owner Network
- Comprehensive Training, Support, and Coaching
- Backed by Five Star Franchising and Princeton Equity
Top 5 Selling Points:
- Fast Startup with Low Initial Investment
- Home-based and Low Overhead
- Multiple Service Lines = Multiple Revenue Streams
- No Experience Needed
- Robust Training and Support
Established: 2006
First Unit Franchised: 2010
Franchised Units: 130+
Company Owned Units: 0
States Registered In: Filed or filling with all registrations states excluding Hawaii (reach out to primary contact for confirmation / status)
Canada Franchises: No
International Franchises: No
Last Updated: May 09, 2025
- Franchise Fee: $60,000
- Recommended Liquid Capital: $60,000+
- Investment Range: $134,645 – $221,095
- Veteran Discount: $9,000 off Franchise Fee (not applicable to commission levels)
- First Responder Discount: $9,000 off Franchise Fee (not applicable to commission levels)
- Royalty: Scaling; Greater of $750 / month (1-12); $1,500 / month (13-24); $2,250 / month (25+) Or 7.5% of Gross Sales
- Item 19: Yes
Bio-One Training (B.O.T.S) / OSHA Safety Standard (Initial Training)
New franchise owners visit our corporate headquarters for comprehensive initial training to effectively prepare you for day-to-day operations.
- Learn how to price, estimate, sell and market your services effectively
- Get to know the chemicals and equipment you’ll use in the field
- Hands-on simulated training to learn firsthand how to service various biohazard jobs
- Observe a service job in real-time (may vary)
- OSHA Safety Standard Training is conducted on a quarterly basis and must be attended by any
- Owner or Designated Manager
Ongoing Support
- Enjoy the flexibility of owning your own business and benefit from an experienced franchise network and trusted support team. A Bio-One family that is experienced, knowledgeable, and willing to help one another.
- Support and ongoing training throughout the life of your agreement
- Train Trainer / Webinars / Town Halls / Regional Meetings / Home Services Summit
- Sharing of systemwide KPI’s, private Facebook groups and frequent internal communication
- Billing specialist who works with insurance adjusters, so you don’t have to
Marketing & Communications
- Onboarding Support
- Website, SEO, PPC, Google Business Profile /OnlineListings, Facebook/Social
- Local Marketing Tools and Resources
- National Branding / Brand Guidelines
- National Sponsorships / Partnerships and Marketing Events
Ideal Candidate
Candidates should enjoy giving back to others and be interested in making a difference in their community. Local networking skills, organization, confidence, and strong work ethic are helpful.
Day In the Life
- Make a daily marketing plan – Decide which locations to market to, how much marketing material to bring, and what your plan of attack is for the day.
- Market – Based on which community you will be marketing to, practice your pitch accordingly. Bring along your little black book of marketing and your business and rack cards.
- Call your team – If you get a call at any time of day, it’s your job to make sure your technicians are ready to arrive on a job site quickly.
- Suit up for a job – When arriving for a job, you should have the proper PPE. We work with chemicals to clean and disinfect dangerous germs and pathogens. Safety is a requirement. ALWAYS.
- Clean a bio job – Each call for a bio job is unique. Make sure you bring the right chemicals and equipment and you’ll be set to get the home back to normal.
- Clean a hoarding job – A typical hoarding job can take a few days to complete. Clean, organize, and sanitize and you’ll be on your way.
- Send an invoice – Price your job reasonably and send your invoice out. Work with your client or their insurance to get paid.
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Submit Candidate Referrals HERE
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Director Contact:
Zach Grimmig (Director of Franchise Development)
Email: zach.grimmig@biooneinc.com
Telephone: 727-253-7170
Director Contact:
Joshua Hoffmann (Director of Franchise Development)
Email: Joshua.hoffmann@biooneinc.com
Telephone: 630-397-9013
VP Contact:
Jacob Hanika (VP, Franchise Development)
Email: jacob.hanika@fivestarfranchising.com
Telephone: 402-690-8847
Corporate Address: 8200 Southpark Circle, Suite 300, Littleton, Colorado 80120
Corporate Website: https://bioonefranchising.com/
Commission Promo: 2025 Five Star Drive Challenge (PDF)
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UNITED STATES REFERRAL FEES:
Single Unit Referral Fee: $40,000
Multiple Unit Referral Fee: $10,000 per unit.
Veteran/First Responder Referral Fee: Same as above
Resale Unit Referral Fee: $30,000 or 10% of the final sale price.